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Test test need to pay attention to which five experimental details
2017/3/20
The success rate of the experiment is very important, and to improve the success rate, we must pay attention to detail. The following is the need to pay attention to the experiment five major details: from Shanghai love heart for your well-edited, thank you for the Shanghai Hengyuan biological support and trust, I wish you a happy shopping!
A record
People's memory is limited, in the experiment, it is impossible to remember every step done. For example, if the experimental record is not written in time, the next day will forget a lot, a week later completely forgotten. So do experiments must do a good job of experimental records. Record a timely, otherwise you will forget, it is best to record at any time. Two to be detailed, because a very small details may be the cause of the experiment failed or successful, if not write down the details of the experiment is difficult to find the problem. For example, which reagent is used as an experimental reagent, and when it is equipped with 5% BSA with PBST plus 0.02% sodium Azide to inhibit microbial growth, it should be recorded as 5 % BSA.PBST.N3Na, but 5% BSA. Three to be neat, if too scrawled, it seems very convenient, need to find relevant information when it is difficult to find. A good label is also a record, the label must be detailed: including the reagent name, configuration date, configuration and other necessary information. In the case of reagents, in order to prevent the forgotten labeling of the situation, should be labeled, plus reagents. Electronic records have the advantage of being easy to retrieve, and for some important information, you can record electronic documents (such as word documents) inside.
Second verification
Very often, the reason my experiment failed was not carefully checked, for example, did not check the phenotype of the fruit fly, did not carefully check the reagent label, did not check the centrifuge speed, did not check the heat block temperature and so on. Every small mistake can lead to experimental failure, so be sure to develop a careful check the habit. Specifically, when adding reagents, be sure to carefully check the label (including the name of the reagent, the date of the configuration), whether there is precipitation, whether the color changes; use the instrument, to check the settings, such as temperature, speed, time settings ; When electrophoresis, to check whether there is air bubbles; use of experimental animals, to check the phenotype and so on.
Three packs
Proverbs: Do not put all the eggs in the same basket. Similarly, when doing the experiment, sometimes do not put all the reagents into the same container. There are a lot of benefits. First, insurance. It is possible to prevent all reagents or samples from being lost due to contamination or deterioration. Second, easy to mix. Icing reagents need all dissolved and then vortex can be mixed, if a large volume, and sometimes can not wait for all dissolved began to add samples, will lead to uneven concentration. The general solution is the first dissolved solute concentration is high, like frozen soda while melting side of the drink, the beginning of sweet, and finally almost no sweet, because the presence of sugar will reduce the melting point, high sugar content First dissolved. After packing, each tube solution is small, easy to dissolve all thawed. Note that when the packaging must be fully dissolved and fully mixed. Third, extend the shelf life. Many reagents should be stored at low temperature. If not sub-installed, each time the use of all the heat once, metamorphosis will soon. After packing, only a small amount of reagents are used each time, the other can continue to keep at low temperature, can extend the service life. Antibodies, larger enzymes, other reagents that are thermally unstable, etc. are preferably stored at a temperature of 20 degrees or less.
Four mix
Experiments on the amount of reagents are clearly required. To ensure the success of the experiment and repeatability, must ensure that the amount of reagent consistent. The amount is equal to the volume multiplied by the concentration, if the solution is not mixed, then the concentration will be different, even if the same volume, the actual amount may not be the same. Therefore, be sure to ensure that the reagent is fully mixed. The icing reagent must be completely dissolved before it can be used. Easy to precipitate the reagents, before use must be fully vortex and then quickly centrifuge to use.
In the book "At the bench", the author said that if the laboratory fire and other critical situations, the first should be rescued is not a reagent, but experimental records. Because the experimental record can complete the paper. In fact, in order to prevent the loss of experimental records, you can scan the experimental record as a PDF document to backup. At the same time, for important research data, it is best to organize electronic documents such as excel or word documents, and develop their own computer files regularly backup habits. In addition, the plasmid, the transformed bacteria also need to be backed up. It is best to back up two to reduce the probability of loss.
A record
People's memory is limited, in the experiment, it is impossible to remember every step done. For example, if the experimental record is not written in time, the next day will forget a lot, a week later completely forgotten. So do experiments must do a good job of experimental records. Record a timely, otherwise you will forget, it is best to record at any time. Two to be detailed, because a very small details may be the cause of the experiment failed or successful, if not write down the details of the experiment is difficult to find the problem. For example, which reagent is used as an experimental reagent, and when it is equipped with 5% BSA with PBST plus 0.02% sodium Azide to inhibit microbial growth, it should be recorded as 5 % BSA.PBST.N3Na, but 5% BSA. Three to be neat, if too scrawled, it seems very convenient, need to find relevant information when it is difficult to find. A good label is also a record, the label must be detailed: including the reagent name, configuration date, configuration and other necessary information. In the case of reagents, in order to prevent the forgotten labeling of the situation, should be labeled, plus reagents. Electronic records have the advantage of being easy to retrieve, and for some important information, you can record electronic documents (such as word documents) inside.
Second verification
Very often, the reason my experiment failed was not carefully checked, for example, did not check the phenotype of the fruit fly, did not carefully check the reagent label, did not check the centrifuge speed, did not check the heat block temperature and so on. Every small mistake can lead to experimental failure, so be sure to develop a careful check the habit. Specifically, when adding reagents, be sure to carefully check the label (including the name of the reagent, the date of the configuration), whether there is precipitation, whether the color changes; use the instrument, to check the settings, such as temperature, speed, time settings ; When electrophoresis, to check whether there is air bubbles; use of experimental animals, to check the phenotype and so on.
Three packs
Proverbs: Do not put all the eggs in the same basket. Similarly, when doing the experiment, sometimes do not put all the reagents into the same container. There are a lot of benefits. First, insurance. It is possible to prevent all reagents or samples from being lost due to contamination or deterioration. Second, easy to mix. Icing reagents need all dissolved and then vortex can be mixed, if a large volume, and sometimes can not wait for all dissolved began to add samples, will lead to uneven concentration. The general solution is the first dissolved solute concentration is high, like frozen soda while melting side of the drink, the beginning of sweet, and finally almost no sweet, because the presence of sugar will reduce the melting point, high sugar content First dissolved. After packing, each tube solution is small, easy to dissolve all thawed. Note that when the packaging must be fully dissolved and fully mixed. Third, extend the shelf life. Many reagents should be stored at low temperature. If not sub-installed, each time the use of all the heat once, metamorphosis will soon. After packing, only a small amount of reagents are used each time, the other can continue to keep at low temperature, can extend the service life. Antibodies, larger enzymes, other reagents that are thermally unstable, etc. are preferably stored at a temperature of 20 degrees or less.
Four mix
Experiments on the amount of reagents are clearly required. To ensure the success of the experiment and repeatability, must ensure that the amount of reagent consistent. The amount is equal to the volume multiplied by the concentration, if the solution is not mixed, then the concentration will be different, even if the same volume, the actual amount may not be the same. Therefore, be sure to ensure that the reagent is fully mixed. The icing reagent must be completely dissolved before it can be used. Easy to precipitate the reagents, before use must be fully vortex and then quickly centrifuge to use.
Five backup
In the book "At the bench", the author said that if the laboratory fire and other critical situations, the first should be rescued is not a reagent, but experimental records. Because the experimental record can complete the paper. In fact, in order to prevent the loss of experimental records, you can scan the experimental record as a PDF document to backup. At the same time, for important research data, it is best to organize electronic documents such as excel or word documents, and develop their own computer files regularly backup habits. In addition, the plasmid, the transformed bacteria also need to be backed up. It is best to back up two to reduce the probability of loss.
